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hiv 1 gp120  (Novus Biologicals)


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    Structured Review

    Novus Biologicals hiv 1 gp120
    Hiv 1 Gp120, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hiv+1+gp120/HIV-1+gp120+Antibody+-+BSA+Free/10__20517_slash_evcna__2025__106-158-90-93
    Average 93 stars, based on 2 article reviews
    hiv 1 gp120 - by Bioz Stars, 2026-09
    93/100 stars

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    Incubation:

    Article Title: Simultaneous Protein and RNA Analysis in Single Extracellular Vesicles, Including Viruses.
    Article Snippet: .. Chips were then incubated with fluorescent antibodies against CD63 (CD63-CF647, from ExoView kit), CD81 (CD81-CF555, from ExoView kit), and HIV-1 gp120 (gp120-AF488, from Novus Biologicals, #NBP3-06630AF488). ..

    Article Title: Simultaneous Protein and RNA Analysis in Single Extracellular Vesicles, Including Viruses.
    Article Snippet: .. Chips were then incubated with fluorescent antibodies against CD81 (CD81-CF555, from ExoView kit) and HIV-1 gp120 (gp120-AF488, from Novus Biologicals, #NBP3-06630AF488), and 2 μL of smFISH probes (from 100 ng/μL smFISH probe stock). ..

    Article Title: Simultaneous protein and RNA analysis in single extracellular vesicles, including viruses: SPIRFISH
    Article Snippet: .. Chips were then incubated with fluorescent antibodies against CD81 (CD81-CF555, from ExoView kit) and HIV-1 gp120 (gp120-AF488, from Novus Biologicals, #NBP3-06630AF488), and 2 μL of smFISH probes (from 100 ng/μL smFISH probe stock). ..

    Article Title: Simultaneous Protein and RNA Analysis in Single Extracellular Vesicles, Including Viruses
    Article Snippet: .. Chips were then incubated with fluorescent antibodies against CD63 (CD63-CF647, from ExoView kit), CD81 (CD81-CF555, from ExoView kit), and HIV-1 gp120 (gp120-AF488, from Novus Biologicals, #NBP3-06630AF488). ..

    Article Title: Simultaneous Protein and RNA Analysis in Single Extracellular Vesicles, Including Viruses
    Article Snippet: .. Chips were then incubated with fluorescent antibodies against CD81 (CD81-CF555, from ExoView kit) and HIV-1 gp120 (gp120-AF488, from Novus Biologicals, #NBP3-06630AF488), and 2 μL of smFISH probes (from 100 ng/μL smFISH probe stock). ..

    Article Title: Simultaneous protein and RNA analysis in single extracellular vesicles, including viruses: SPIRFISH
    Article Snippet: .. Chips were then incubated with fluorescent antibodies against CD63 (CD63-CF647, from ExoView kit), CD81 (CD81-CF555, from ExoView kit), and HIV-1 gp120 (gp120-AF488, from Novus Biologicals, #NBP3-06630AF488). ..

    Bioprocessing:

    Article Title: HIV protein Nef expression in human microglia drives the release of distinct Nef-containing extracellular vesicles
    Article Snippet: Additionally, 25-30 μg of CL, total EV proteins or proteins from TCAprecipitated EV fractions were separated by 4%-12% Bis-Tris SDS polyacrylamide gel electrophoresis (SDSPAGE), (NuPAGE, Thermo Fisher Scientific, USA) and transferred to the poly(vinylidene fluoride) (PVDF) membrane (Merck Millipore, USA). .. Primary antibodies used were as follows: mouse monoclonal antibodies against AChE (MAB303, Millipore, USA), Alix (2171, Cell Signaling Technology, USA), CD81 (NBP1-44861, Novus Biologicals; USA), Cytochrome C (556433, BD Biosciences, USA), Flotillin (610820, BD Biosciences, USA), GAPDH (G8795, Sigma-Aldrich, USA), GFP (sc-9996, Santa Cruz Biotechnology, USA), HIV-1 Nef (ab42355, UK), Hsp70 (ab5442, Abcam, UK), p24 (ab9071, Abcam, UK) and Tsg101 [4A10] (ab83, Abcam, UK); or goat polyclonal antibodies against Actin (sc-1615, Santa Cruz Biotechnology, USA), Annexin A2 (sc-1924) and HSC70 (sc1059); or rabbit polyclonal antibodies against Calnexin (sc-11397), CD63 (sc-15363), HIV-1 gp120 (NBP176371, Novus Biologicals, USA) and HIV-1 Nef (2949, BEI Resources Repository, USA); and rabbit monoclonal antibodies against Albumin (ab192603, Abcam, UK) and CD9 (13403, Cell Signaling Technology, USA). .. Horseradish peroxidase (HRP)-conjugated secondary antibodies (anti-mouse, anti-goat, and anti-rabbit; Jackson ImmunoResearch Laboratories, USA) were used for detection.

    Article Title: HIV protein Nef expression in human microglia drives the release of distinct Nef-containing extracellular vesicles
    Article Snippet: Additionally, 25-30 μg of CL, total EV proteins or proteins from TCA-precipitated EV fractions were separated by 4%-12% Bis-Tris SDS polyacrylamide gel electrophoresis (SDS-PAGE), (NuPAGE, Thermo Fisher Scientific, USA) and transferred to the poly(vinylidene fluoride) (PVDF) membrane (Merck Millipore, USA). .. Primary antibodies used were as follows: mouse monoclonal antibodies against AChE (MAB303, Millipore, USA), Alix (2171, Cell Signaling Technology, USA), CD81 (NBP1-44861, Novus Biologicals; USA), Cytochrome C (556433, BD Biosciences, USA), Flotillin (610820, BD Biosciences, USA), GAPDH (G8795, Sigma-Aldrich, USA), GFP (sc-9996, Santa Cruz Biotechnology, USA), HIV-1 Nef (ab42355, UK), Hsp70 (ab5442, Abcam, UK), p24 (ab9071, Abcam, UK) and Tsg101 [4A10] (ab83, Abcam, UK); or goat polyclonal antibodies against Actin (sc-1615, Santa Cruz Biotechnology, USA), Annexin A2 (sc-1924) and HSC70 (sc-1059); or rabbit polyclonal antibodies against Calnexin (sc-11397), CD63 (sc-15363), HIV-1 gp120 (NBP1-76371, Novus Biologicals, USA) and HIV-1 Nef (2949, BEI Resources Repository, USA); and rabbit monoclonal antibodies against Albumin (ab192603, Abcam, UK) and CD9 (13403, Cell Signaling Technology, USA). .. Horseradish peroxidase (HRP)-conjugated secondary antibodies (anti-mouse, anti-goat, and anti-rabbit; Jackson ImmunoResearch Laboratories, USA) were used for detection.



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    Image Search Results


    a , Antibody response dynamics measured as plasma reactivity against HIV-1 Env proteins (gp120, pink; gp140, green) and pseudovirus-neutralizing activity (autologous pseudovirus, orange; Ba-L clade B pseudovirus, blue). pNT, pseudovirus neutralization titre. b , Virus-specific CD8 T cell responses (measured as production of interferon-γ (IFNγ), tumour necrosis factor (TNF) or interleukin-2 (IL-2)) against CMV (pink), EBV (green), HHV-6 (purple) and HIV (light blue) peptide pools at 60, 72 and 81 months after ART interruption. Staphylococcus enterotoxin B (SEB, black) represents the positive control.

    Journal: Nature

    Article Title: Sustained HIV-1 remission after heterozygous CCR5Δ32 stem cell transplantation

    doi: 10.1038/s41586-025-09893-0

    Figure Lengend Snippet: a , Antibody response dynamics measured as plasma reactivity against HIV-1 Env proteins (gp120, pink; gp140, green) and pseudovirus-neutralizing activity (autologous pseudovirus, orange; Ba-L clade B pseudovirus, blue). pNT, pseudovirus neutralization titre. b , Virus-specific CD8 T cell responses (measured as production of interferon-γ (IFNγ), tumour necrosis factor (TNF) or interleukin-2 (IL-2)) against CMV (pink), EBV (green), HHV-6 (purple) and HIV (light blue) peptide pools at 60, 72 and 81 months after ART interruption. Staphylococcus enterotoxin B (SEB, black) represents the positive control.

    Article Snippet: Wells were either coated overnight at 4 °C with 10 μg ml –1 HIV-1 Ba-L_01 gp120 protein (Sino Biological) or left uncoated (PBS only, as control).

    Techniques: Clinical Proteomics, Activity Assay, Neutralization, Virus, Positive Control